The next year, PacBio developed a new method to allow multiple sequencing passes on the same DNA molecule. Double stranded DNA templates were ligated to two single stranded adapters, creating what the company called a “SMRTbell template.” Sequencing began at a primer on one of the adaptors and could proceed multiple times per molecule due to circularization, in a process called rolling-circle amplification. This helped reduce PacBio’s error rates significantly.
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have to do some bookkeeping in order to avoid variable name clashes and so forth. You also wouldn’t allocate a new tuple，更多细节参见快连下载-Letsvpn下载
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